Biotechnologia Acta


  • Increase font size
  • Default font size
  • Decrease font size
Home Archive 2021 № 5 ANTIBIOTIC RESISTANCE OF LACTIC ACID BACTERY LEAVEN «VIVO PROBIOYOGURT» I. M. Korniienko, L. S. Yastremska, L. Y. Polonchuk, M. M. Baranovskyi
Print PDF

ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

Biotechnologia Acta  Т. 14, No. 5 , 2021
P.  63 ˗ 73, Bibliography 26 , Engl.
UDC: 579.6.086.83:577


I. M. Korniienko, L. S. Yastremska, L. Y. Polonchuk, M. M. Baranovskyi

National Aviation University, Kyiv, Ukraine

Lactic acid bacteria play a key role in human microecology and biotechnology – form organoleptic characteristics of products, increase the nutritional, including biological value of functional foods. Natural resistance to antibiotics is one of the important factors that determine the probiotic properties of lacto- and bifidobacteria.

Aim. Study of the antibiotic resistance of functionally active probiotic cultures of "VIVO probioyogurt" leaven to determine the possibility of using a fermented milk product, which is prepared on its basis, during antibiotic therapy to maintain and restore normal intestinal microflora.

Methods. Pure cultures of lactic acid bacteria (LAB) were selected for the study: (Lactobacillus delbrueckii ssp., L. acidophilus, L.casei, L. rhamnosus, L.paracasei, Streptococcus thermophilus, Bifidobacterium lactis (2 strains), B. infantis), which are part of leaven "VIVO probioyogurt" the quality of which is confirmed by certificates of the International Organization for Standardization ISO 9001: 2008, as well as ISO 22000: 2005. The method of the experiment consisted of the following stages: preparation of nutrient media ("Lactobacagar", "Bifidoagar", glucose-peptone medium), working solutions of antibiotics; working suspension of LAB; suspensions of cultures (lacto- and bifidobacteria), cultivation LAB on elective nutrient media with the addition of antibiotics and evaluation of research results. Determination of antibiotic resistance of LAB was performed by the method of double dilutions.

Results. The use of this technique enabled to establish the minimum inhibitory concentration (MIC) of antibiotics of different groups relative to the LAB. The results of the research were processed using a licensed computer program Microsoft Excel.

Conclusions. Evaluation of the results of studies to determine the MIC of antibiotics – benzylpenicillin, azithromycin, lincomycin, gentamicin sulfate, ceftriaxone, norfloxacin, amoxil, streptomycin, tetracycline, erythromycin in relation to IBD; fermented milk product, which was prepared on the basis of this starter culture, it was advisable to use during antibiotic therapy to restore and maintain normal intestinal microflora.

Key words: antibiotic resistance, lactic acid bacteria, minimum inhibitory concentration, yeast, probiotics.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2021

  • References
    • 1. Neposhyvaylenko N., Kornienko I. Current problems of individual health of adolescents and the use of modern food biotechnology to solve them. Collective Monograph: Actual problems of natural sciences: modern scientific discussions. Lublin: Universiti of life sciences in Lublin. 2020, P. 391–409.

      2. Cherniak L., Mikhyeyev O., Madzhd S., Lapan O., Dmytrukha T., Kornienko I. The Usage of Plant Test Systems for the Determination of Phytotoxicity of Contaminated with Petroleum Products Soil. J. Ecol. Eng. 2021, 22 (6), 66–71.

      3. Omelych I., Neposhyvailenko N., Zberovskyi O., Kornienko I. Improvement of the methodology for the assessment of soil biogenig pollution through the use of geological approaches and the use of information technologies. Eastern-European J. Enterpise Technol. 2021, 3 (10 (111)), 42–56.

      4. Alvira P., Tomas-Pejo E., Ballesteros M., Negro M.J. Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis: a review. Bioresour. Technol. 2010, 101, 4851–4861.

      5. Shenderov B. A. Medical microbial ecology and functional nutrition. Probiotics and functional nutrition. Moskva: Grant. 2001, 287 р.

      6. Ganina V. I. Probiotics. Purpose, properties and bases of biotechnology. Moskva: MGUPB. 2001, 169 p.

      7. Novik G. I., Samartsev A. A., Astapovich N. I., Kavrus M. A., Mikhaliuk A. N. Biological activity of probiotic microorganisms. Appl. Biochem. Microbiol. 2006, 42 (2), 166–172.

      8. Samoilov V. A., Nesterenko P. G., Suyunchev O. A. Probiotic lactic acid products. Dairy industry. 2007, V. 7, P. 45–47.

      9. Shenderov B. A. Probiotics and functional nutrition. Moskva: Grant. 2001, 288 p.

      10. Vinogradskaya S. E. Study of the sensitivity of lactic acid cultures and microflora of fermented milk products to antibiotics. Collection of scientific works of SevKavGTU, Series "Food". 2005, V. 1, P. 19–23.

      11. Biofortification and functional products based on the Russian Empire for 2012–2016: the concept of state scientific and technical programs. URL: (date of the blast: 06/10/2021).

      12. Concept of functional food. StudFiles: student file archive. URL: (date of the blast: 06/10/2021).

      13. State and development prospects of the functional food products market. Online student library. URL: (date of the blast: 06/10/2021).

      14. KMU. Decree from 24 March 2021 p. № 305 "About the consolidated norms and the Order of organizing food for the pledges of education and child pledges of health improvement and recovery" [Electronic resource]. 2021. Mode of access to the resource:

      15. Karapetyan K. J. Comparative evaluation of a number of properties of new strains of 93 lactic acid bacteria. Biol. J. Armenia. 2009, 4 (61), 36–42.

      16. Kitaevskaya S. V. Resistance of probiotic strains of lactic acid bacteria to antibiotics. Bulletin of Kazan Technol. Un-ty. 2012, 21 (15), 108–110.

      17. Shchetko V. A., Golovneva N. A. Sensitivity of bifidobacteria to antibiotics of various classes. News of the National Academy of Sciences of Belarus. 2014, V. 2, 103–107.

      18. Sukhorukova M. V. Antibiotic susceptibility of bacterial strains included in the linex probiotic. Clin. Microbiol. Antimicrobial Chemother. 2012, 3 (14), 245–251.

      19. Determination of the sensitivity of microorganisms to antibacterial drugs: guidelines. Approved. G. G. Onishchenko. Moskva: Federal Center for State Sanitary and Epidemiological Supervision of the Ministry of Health of Russia. 2004, 91 p.

      20. Savitskaya I. S., Zhubanova A. A., Kistaubaeva A. S., Bolekbaeva A. B. Antibiotic resistance of lactobacilli – probiotics. KazNU Bulletin. Biol. series. 2017, 56 (4), 222–227.

      21. Bagdasaryan A. S., Tokaev E. S., Nekrasov E. A., Oleynik E. A. Antibiotic resistance of probiotic cultures included in the synbiotic. Proceedings of higher educational institutions. Food Technol. 2011, V. 2–3, P. 102–104.

      22. Plotnikova D. T., Sidorenko A. V., Novik G. I. Study of antibiotic resistance of bacteria of the genera Lactococcus, Enterococcus, Leuconostoc. Vesti National Academy of Sciences of Belarus. 2016, V. 3, P. 94–100.

      23. Lysak V. A, Zheldakova R. A., Fomina O. V. Microbiology. Workshop: manual. Minsk: BSU. 2015, 115 p.

      24. DEN-1 Densitometer [Electronic resource]. Biosan Medical-Diological Research & Technologies. Access mode to the resource:

      25. Кorniienko I. M.,. Filimonenko O. Y., Kriukovska O. A., Hedzun Е. О., Hlushkov A. S. Research of efficiency of application of api-products in practice of preparation of biologically active functional lactic acid products. Collection of scientific works of the Dnieper State Technical University. 2019, 1 (34), 108–112.

      26. Korniienkо I., Lutsenko O., Isaienko V., Baranovskyi M., Anatskyi A., Laricheva L. Optimization of technological parameters of nutrition mixture fermentation process with the use of spline interpolation. Chem. Technol.. 2021, 29 (1), 118136.


И. В. Косаковская, В. А. Васюк
Институт ботаники им. Н. Г. Холодного НАН Украины, Киев
Е-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it , This e-mail address is being protected from spambots. You need JavaScript enabled to view it
Вступление. Гиббереллины (ГБ), класс дитерпеноидных фитогормонов, играют важную роль в регуляции роста и развития растений. Среди более чем 130 изоформ гормона лишь немногие обладают биологической активностью. ГК1, ГК3, ГК4 и ГК7 регулируют рост, влияя на деградацию DELLA протеинов – негативных регуляторов генов, экспрессия которых активируется ГБ. Недавние исследования в области молекулярной генетики и функциональной геномики существенно расширили наши представления о биосинтезе, метаболизме, транспортировке, сигналинге и взаимодействии ГБ с другими фитогормонами и факторами окружающей среды. Цель. В обзоре сосредоточено внимание на роли ГБ в регуляции роста и развития растений при действии абиотических стрессов. Результаты. Приведена ключевая информация о биосинтезе, сигналинге и функциональной активности ГБ, обобщены сведения о перекрестном взаимодействии (кросс-ток) между ГБ, ауксинами, цитокининами, абсцизовой кислотой и другими фитогормонами, о роли ГБ в адаптации к условиям засухи, засоления, высокой и низкой температуры, загрязнения тяжелыми металлами. Эффекты ГТ зависят в первую очередь от интенсивности и продолжительности стрессового воздействия, а также от фазы онтогенеза и толерантности растения. Изменяя интенсивность биосинтеза, характер распределения и передачи сигналов ГБ, растения могут регулировать устойчивость к абиотическому стрессу, повышать жизнеспособность и даже избегать стресса. Обсуждены вопросы использования ретардантов – ингибиторов биосинтеза ГБ с целью изучения функциональной активности гормона в условиях абиотических стрессов. Особое внимание уделено новым биотехнологическим подходам, которые используют экзогенные ГБ для предпосевного праймирования семян и фолиарной обработки растений. Выводы. Дальнейшее изучение роли гиббереллинов в приобретении стрессоустойчивости будет способствовать развитию биотехнологии экзогенного использования гормона для улучшения роста и повышения урожайности растений в неблагоприятных условиях окружающей среды.
Ключевые слова: гиббереллины, DELLA, фитогормоны, абиотические стрессы, ретарданты, рост, стрессоустойчивость.
© Институт биохимии им. А. В. Палладина НАН Украины, 2021


Additional menu

Site search

Site navigation

Home Archive 2021 № 5 ANTIBIOTIC RESISTANCE OF LACTIC ACID BACTERY LEAVEN «VIVO PROBIOYOGURT» I. M. Korniienko, L. S. Yastremska, L. Y. Polonchuk, M. M. Baranovskyi

Invitation to cooperation

Dear colleagues, we invite you to publish your articles in our journal.
© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2008.
All rights are reserved. Complete or partial reprint of the journal is possible only with the written permission of the publisher.
for information: