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Home Archive 2021 № 5 ANTIBIOTIC RESISTANCE OF LACTIC ACID BACTERY LEAVEN «VIVO PROBIOYOGURT» I. M. Korniienko, L. S. Yastremska, L. Y. Polonchuk, M. M. Baranovskyi
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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

Biotechnologia Acta  Т. 14, No. 5 , 2021
P.  63 ˗ 73, Bibliography 26 , Engl.
UDC: 579.6.086.83:577
https://doi.org/10.15407/biotech14.05.063

ANTIBIOTIC RESISTANCE OF LACTIC ACID BACTERY LEAVEN «VIVO PROBIOYOGURT»

I. M. Korniienko, L. S. Yastremska, L. Y. Polonchuk, M. M. Baranovskyi

National Aviation University, Kyiv, Ukraine

Lactic acid bacteria play a key role in human microecology and biotechnology – form organoleptic characteristics of products, increase the nutritional, including biological value of functional foods. Natural resistance to antibiotics is one of the important factors that determine the probiotic properties of lacto- and bifidobacteria.

Aim. Study of the antibiotic resistance of functionally active probiotic cultures of "VIVO probioyogurt" leaven to determine the possibility of using a fermented milk product, which is prepared on its basis, during antibiotic therapy to maintain and restore normal intestinal microflora.

Methods. Pure cultures of lactic acid bacteria (LAB) were selected for the study: (Lactobacillus delbrueckii ssp., L. acidophilus, L.casei, L. rhamnosus, L.paracasei, Streptococcus thermophilus, Bifidobacterium lactis (2 strains), B. infantis), which are part of leaven "VIVO probioyogurt" the quality of which is confirmed by certificates of the International Organization for Standardization ISO 9001: 2008, as well as ISO 22000: 2005. The method of the experiment consisted of the following stages: preparation of nutrient media ("Lactobacagar", "Bifidoagar", glucose-peptone medium), working solutions of antibiotics; working suspension of LAB; suspensions of cultures (lacto- and bifidobacteria), cultivation LAB on elective nutrient media with the addition of antibiotics and evaluation of research results. Determination of antibiotic resistance of LAB was performed by the method of double dilutions.

Results. The use of this technique enabled to establish the minimum inhibitory concentration (MIC) of antibiotics of different groups relative to the LAB. The results of the research were processed using a licensed computer program Microsoft Excel.

Conclusions. Evaluation of the results of studies to determine the MIC of antibiotics – benzylpenicillin, azithromycin, lincomycin, gentamicin sulfate, ceftriaxone, norfloxacin, amoxil, streptomycin, tetracycline, erythromycin in relation to IBD; fermented milk product, which was prepared on the basis of this starter culture, it was advisable to use during antibiotic therapy to restore and maintain normal intestinal microflora.

Key words: antibiotic resistance, lactic acid bacteria, minimum inhibitory concentration, yeast, probiotics.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2021

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ГИББЕРЕЛИНЫ В РЕГУЛЯЦИИ РОСТА И РАЗВИТИЯ РАСТЕНИЙ
В УСЛОВИЯХ АБИОТИЧЕСКИХ СТРЕССОВ
И. В. Косаковская, В. А. Васюк
Институт ботаники им. Н. Г. Холодного НАН Украины, Киев
Е-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it , This e-mail address is being protected from spambots. You need JavaScript enabled to view it
Вступление. Гиббереллины (ГБ), класс дитерпеноидных фитогормонов, играют важную роль в регуляции роста и развития растений. Среди более чем 130 изоформ гормона лишь немногие обладают биологической активностью. ГК1, ГК3, ГК4 и ГК7 регулируют рост, влияя на деградацию DELLA протеинов – негативных регуляторов генов, экспрессия которых активируется ГБ. Недавние исследования в области молекулярной генетики и функциональной геномики существенно расширили наши представления о биосинтезе, метаболизме, транспортировке, сигналинге и взаимодействии ГБ с другими фитогормонами и факторами окружающей среды. Цель. В обзоре сосредоточено внимание на роли ГБ в регуляции роста и развития растений при действии абиотических стрессов. Результаты. Приведена ключевая информация о биосинтезе, сигналинге и функциональной активности ГБ, обобщены сведения о перекрестном взаимодействии (кросс-ток) между ГБ, ауксинами, цитокининами, абсцизовой кислотой и другими фитогормонами, о роли ГБ в адаптации к условиям засухи, засоления, высокой и низкой температуры, загрязнения тяжелыми металлами. Эффекты ГТ зависят в первую очередь от интенсивности и продолжительности стрессового воздействия, а также от фазы онтогенеза и толерантности растения. Изменяя интенсивность биосинтеза, характер распределения и передачи сигналов ГБ, растения могут регулировать устойчивость к абиотическому стрессу, повышать жизнеспособность и даже избегать стресса. Обсуждены вопросы использования ретардантов – ингибиторов биосинтеза ГБ с целью изучения функциональной активности гормона в условиях абиотических стрессов. Особое внимание уделено новым биотехнологическим подходам, которые используют экзогенные ГБ для предпосевного праймирования семян и фолиарной обработки растений. Выводы. Дальнейшее изучение роли гиббереллинов в приобретении стрессоустойчивости будет способствовать развитию биотехнологии экзогенного использования гормона для улучшения роста и повышения урожайности растений в неблагоприятных условиях окружающей среды.
Ключевые слова: гиббереллины, DELLA, фитогормоны, абиотические стрессы, ретарданты, рост, стрессоустойчивость.
© Институт биохимии им. А. В. Палладина НАН Украины, 2021

 

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Home Archive 2021 № 5 ANTIBIOTIC RESISTANCE OF LACTIC ACID BACTERY LEAVEN «VIVO PROBIOYOGURT» I. M. Korniienko, L. S. Yastremska, L. Y. Polonchuk, M. M. Baranovskyi

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