Biotechnologia Acta


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IISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

Biotechnologia Acta V. 14, No 2, 2021
Р.  59-66, Bibliography 24 , English
Universal Decimal Classification:[577.13+543.645]:581.143.6


M. O. Twardovska 1, I. I. Konvalyuk 1, K. V. Lystvan 2, I. O. Andreev 1, V. A. Kunakh 1

1 Institute of Molecular Biology and Genetics of the National Academy of Sciences of Ukraine, Kyiv
2 Institute of Cell Biology and Genetic Engineering of the National Academy of Sciences of Ukraine, Kyiv

Aim. The aim of the study was to determine the quantitative and qualitative content of phenolic compounds and flavonoids in Deschampsia antarctica E. Desv. tissue cultures obtained from plants originating from different islands of the maritime Antarctic.

Methods. In vitro tissue culture, Folin-Ciocalteu method, spectrophotometry, HPLC analysis.

Results. The quantitative content of phenolic compounds and flavonoids in D. antarctica tissue cultures obtained from plants of six genotypes (DAR12, DAR13, G/D12-2a, Y66, R30 and L57) was determined. The highest content of phenolic compounds (4.46 and 3.75 mg/g) was found in tissue cultures obtained from root and leaf explants of plant genotype L57. The highest amount of flavonoids (7.17 mg/g) was accumulated in G/D12-2a tissue culture of root origin. The content of the studied biologically active compounds (BACs) did not change with increasing number of subculture generations (from passage 10 to 19). HPLC analysis showed that in D. antarctica tissue cultures, a shift in the biosynthesis of BACs occurred towards the synthesis of more polar metabolites compared to explant donor plants.

Conclusions. It was found that the transition of cells to undifferentiated growth affected the content of BACs, the amount of which decreased 2–5 times simultaneously with a significant change in their profile. This provided a basis for further biochemical studies, as well as for careful selection of tissue culture of D. antarctica to use it as a potential source of BACs.

Key words: plant tissue culture, Deschampsia antarctica E. Desv., phenolic compounds, flavonoids, HPLC analysis.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2021

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