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Home Archive 2021 № 1 THE EFFECTIVENESS OF PHYTOLECTINS AND LECTIN COMPOSITIONS APPLICATION FOR SPRAYING PLANTS DURING VEGETATION Kyrychenko O. V., Kots S. Ya., Pukhtaievych P. P.
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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

EnglishBiotechnologia Acta V. 14, No 1, 2021/
Р. 57-68, Bibliography  20,
Universal Decimal Classification:  581.192: 581.557
https://doi.org/10.15407/biotech14.01.57

THE EFFECTIVENESS OF PHYTOLECTINS AND LECTIN COMPOSITIONS APPLICATION FOR SPRAYING PLANTS DURING VEGETATION

Kyrychenko O. V., Kots S. Ya., Pukhtaievych P. P.

Institute of Plant Physiology and Genetics of the National Academy of Sciences of Ukraine

The effectiveness of the soybean plants spraying with the soybean seed lectin solution during vegetation (against the background of seed inoculation with nodule bacteria and without seed inoculation), as well as the effectiveness of the winter wheat plants spraying with lectin-bacterial composition in green-house and field experiments was investigated respectively.  It was found that spraying of soybeans in the phase of two trifoliate leaves development with a specific lectin against the background of pre-sowing seed inoculation with rhizobia caused a significantly positive effect on the functional activity of the symbiotic apparatus. The nitrogen-fixing activity of the rhizosphere microbiota remained unchanged, which may indicate the vector of lectin action when sprayed through the plant. At the same time, the activation of plants vegetative growth was noted, which was maximally manifested by the height of their above ground part. The activity of exogenous sprayed lectin was less pronounced on the background of seed inoculation with rhizobia compared to non-inoculated plants. Plants spraying with soybean lectin against the background of seed inoculation provided an increase in harvest compared to non-inoculated control by 2.13 g/plant, but by the factor of lectin action this increase was only 0.19 g/plant and was insignificant. Non-inoculated soybean plants when sprayed with lectin formed a harvest that was significantly higher (by 0.64 g/plant) than that of plants in the absence of lectin. At this, the increase by the factor of lectin action was 22%. The spraying of winter wheat plants in the phase of mass spring germinations with the Azolec preparation (without pre-sowing seed inoculation) contributed to a significant increase in harvest by 1.6 c/ha. Therefore, the application of soybean and wheat plants spraying, respectively, with soybean seed lectin and lectin-bacterial Azolec preparation (wheat lectin),without involving pre-sowing seed inoculation, provided a greater degree of plants productive potential realization compared to control (without pre-sowing seed inoculation and plants spraying during vegetation).

Key words: soybean, rhizobia, soybean seed lectin, spraying, inoculation, nitrogen fixation, winter wheat, Azolec preparation, productivity.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2020

  • References
    • 1. Sharon N. Lectins: carbohydrate-specific reagents and biological recognition molecules. J. Biol. Chem. 2007, 282 (5), 2753–2764. https://doi.org/10.1074/jbc

      2. Van Damme E. J. M., Lannoo N., Peumans W. J. Plant Lectins. Adv. Botanical Res. 2008, V. 48, P. 107–209. https://doi.org/10.1016/S0065-2296(08)00403-5

      3. Hamid R., Masood A., Wani I. H., Rafiq S. Lectins: Proteins with diverse applications. J. Appl. Pharm. Sci. 2013, V. 3, P. 93–103. https://doi.org/10.7324/JAPS.2013.34. S18

      4. Lagarda-Diaz I., Guzman-Partida A., VazquezMoreno L. Legume lectins: proteins with diverse applications. Int. J. Mol. Sci. 2017, 18 (6), 1242. https://doi.org/10.3390/ijms18061242

      5. Coelho L. C. B. B., Silva P. M. S., Lima V. L. M., Pontual E. V., Paiva P. M. G., Napoleao Th. H., Correia M. T. S. Lectins, interconnecting proteins with biotechnological/ p h a r m a c o l o g i c a l a n d t h e r a p e u t i c application. Evid. Based Complement Alter. Med. 2017, V. 2017. https://doi.org/10.1155/2017/1594074

      6. Mishra А., Behura А.,Mawatwal Sh., Kumar А., Naik L., Mohanty S. S., Manna D., Dokania Р., Mishra А., Patra S. К., Dhiman R. Structure-function and application of plant lectins in disease biology and immunity. Food Chem. Toxicol. 2019, V. 134, P. 110827. https://doi.org/10.1016/j. fct.2019.110827

      7. Kyrychenko O. V. Phytolectins and Diazotrophs are the polyfunctional components of the complex biological compositions. Biotechnol. acta. 2014, 7 (1), 40–53. (In Ukrainian). https://doi.org/10.15407/biotech7.01.040

      8. Jiang S., Ma Z., Ramachandran S. Evolutionary history and stress regulation of the lectin superfamily in higher plants. BMC Evol. Biol. 2010, 10 (1), 79–103. https://doi.org/10.1186/1471-2148-10-79

      9. Kovalchuk N. V., Melnykova N. M., Musatenko L. I. Role of phytolectin in the life cycle of plants. Biopolymers and cell. 2012, 28 (3), 171–180. https://doi.org/10.7124/bc.00004A

      10. Kandelinskaya O. L., Grischenko E. R., Ripinskaya K. Ju., Aleschenkova Z. M., Kartizhova L. E., Kuptsov V. N., Kup tsov N. S. Role of lectins in regulation of legumerhizobium symbiosis efficiency in lupin. Botanika (issledovaniya). Sb. Nauch. Tr.: In-t Experimental noy Botaniki NAN Belarusi. Minsk: In-t radiobiologii. 2015, No 44, P. 283–290. (In Russian).

      11. Pavlovskaya N. E., Gagarina I. N. The physiological properties of plant lectins as a prerequisite for their application in biotechnology. Khimiya rastitelnogo syriya. 2017, No 1, P. 21–35. (In Russian). https://doi.org/10.14258/jcprm.2017011298

      12. Pavlovskaya N. E., Gagarina I. N., Rogovin V. V., Borzenkova G. A., Mushtakova V. M., Fomina V. A. Means for presowing treatment of pea seeds: Pat. Rus. No 2372763. Оpubl. 20. 11. 2009, Byul. No 33. (In Russian).

      13. Sytnikov D. M. Economic effect and application of rhizobial preparations modified with homologous lectins. Microbiol. Biotechnol. 2012, 1 (17), 75–84. (In Russian). https://doi.org/10.18524/2307-4663.2012.1(17).93385

      14. Sergienko V. G., Kyrychenko O. V., Perkovs ka G. Yu. Method of using plant lectins to protect vegetable crops from diseases. Pat. nа kоrysnu model UA No 41723A01N 63/00, A01C 1/06/. Zayavnyk i patentovlasnyk Institute of Plant Protection of Ukrainian Academy of Agrarian Sciences. u200812612. Zayavl. 28. 10. 2008, Оpubl. 10. 06. 2009, Byul. No 11. (In Ukrainian).

      15. Erohin A. I. The effectiveness of the preparation on the basis of lectins on leguminous crops in presowing treatment of seeds and vegetating pea plants. Zernobobovyye i krupyanyye kul’tury. 2019, 2 (30), 48–53. https://doi. org/10.24411/2309-348X-2019-11087

      16. Erohin A. I., Pavlovskaya N. E. Efficacy of combined application of preparations for pea seeds. Zemledelie. 2016, No 4, P. 17–19. (In Russian).

      17. State register of plant varieties suitable for dissemination in Ukraine in 2018. Kyiv: Ministerstvo Ahrarnoi Polityky ta Prodovolstva Ukrainy. 2018, 447 p. www. sops.gov.ua/uploads/page/5aa63108e441e. pdf (In Ukrainian).

      18. Hardy R. W. F., Burns R. C., Holsten R. D. Application of the acetylene-ethylene assay for measurement of nitrogen fixation. Soil. Biol. Biochem. 1973, 5 (1), 41–83. https:// www.ncbi.nlm.nih.gov/pmc/articles/ PMC1086994, https://doi.org/10.1016/0038-0717(73)90093-X

      19. Thandiwe Nleye, Peter Sexton, Kyle Gustafson, Janet Moriles Miller. Soybean Growth Stages. In book: Grow Soybean: Best management Practices for Soybean Production. 2019, Ch. 3, P. 1–11. www. researchgate.net

      20. Sytnikov D. M., Kots S. Ya., Malichenko S. M., Kirizii D. A. Photosynthetic rate and lectin activity of soybean leaves after inoculation with rhizobia together with homologous lectin. Rus. J. Plant Physiology. 2006, 53 (2), 169–175. (In Russian). https://doi.org/10.1134/S102144370602004X


 

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Home Archive 2021 № 1 THE EFFECTIVENESS OF PHYTOLECTINS AND LECTIN COMPOSITIONS APPLICATION FOR SPRAYING PLANTS DURING VEGETATION Kyrychenko O. V., Kots S. Ya., Pukhtaievych P. P.

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