Select your language

ISSN 2410-776X (Online),
ISSN 2410-7751 (Print)

 5 2013

"Biotechnologia Acta" v. 6, no. 5, 2013
https://doi.org/10.15407/biotech6.05.131
Р.131-136, Bibliography15, Russian
Universal Decimal classification: 616.71-007.237-053.2:616-018.2-008.9

METABOLIC CHANGES OF CONNECTIVE TISSUE IN CHILDREN WITH BONE CYST

O. M. Magomedov1, A. F. Levytsky2, D. V. Golovatiuk3, A. N. Velikyy3, T. A. Polishchuk1

1SI «Institute for Orthopedics and Traumatology of National Academy of Medical Sciences of Ukraine», Kyiv, Ukraine
2Bogomolets National Medical University, Kyiv, Ukraine
3National Pediatric Specialized Hospital «OKHMATDYT», Kyiv, Ukraine

The results of the study of diagnostically important metabolism parameters in patients with bone cysts in different stages of the disease are presented. It is shown that an increase activity of protein banding collagenase, alkaline phosphatase and also of hydroxyproline, glycosaminoglycans contents due to lower levels of calcium and inorganic phosphate levels increase in blood serum are expressed in a stage osteolysis than the step of separating. Decreasing the amount of glycosaminoglycans and collagen in bone indicates an intensification of catabolic processes in the connective tissue matrix.

Diagnostically important indicators of the degree of disturbance of bone metabolism are the level of collagen, proteoglycans and activity of marker enzymes — collagenase and alkaline phosphatase. Based on the evaluation of sensitivity, specificity and diagnostic efficiency of the obtained results, we can recommend the threshold values of the investigated parameters of basic organic components and mineral metabolism of bone for the differential diagnosis of stages of bone cysts in children, which will serve as a basis for the development of appropriate diagnostic tests.

Key words: metabolic indices, connective tissue, bone cysts.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2013

References

1. Fletcher C. D. M., Unni K. K., Mertens F. (Eds.): World Health Organization Classification of Tumours. Pathology and Genetics of Tumours of Soft Tissue and Bone. IARC Press: Lyon. 2002.

2. Vibornov D. Yu. Degenerative bone cysts in children (morphogenesis, clinical features, diagnosis and treatment): Dis. ... dr. med. sciences. Мoskva. 2004, 250 p. (In Russian).

3. Sholokhova N. A. Surgical treatment of benign tumors and tumor-like bone diseases inside and periarticular localization in children and adolescents. Abstract dis. ... cand. honey. sciences. Мoskva. 2010, 23 p. (In Russian).

4. Stogov M. B., Lunyeva S. N., Mitrofannov A. I., Tkachuk E. A. Features of the chemical composition of the contents of bone cysts, depending on the stage of development. Klin. lab. diagnostic. 2012, N 11, P. 21–22. (In Russian).

5. Lunyeva S. N., Stogov M. B., Mitrofannov A. I. chemical composition of the contents of solitary bone cysts in the combined treatment with perosseous osteosynthesis. Vestn. ortop. travmatol. im. N. I. Priorova. 2011, N 3, P. 33–37. (In Russian).

6. Slutskiy L. I. Biochemistry of normal and pathological changes of the connective tissue. Leningrad, Meditsina. 1969, P. 378. (In Russian).

7. Kuftyrev L. M., Luneva S. N., Pozharishchenskiy E. K. The genius of orthopedics. Kurgan. 2002, N 1, P. 77–80. (In Russian).

8. Magomedov S., Korpan M. I., Brusko A. T. Visn. ortop. travmatol. protez. 2011, N 3, P. 74–80.

9. Frey S. Etude d’une method d’exploration et du taux normal de l’hydroxyproline du serum. Biochem. Biophys. Ets. 1965, 3 (2), 446–450.

10. Stegemann H. J. A simple procedure for the determination of hydroxyproline in urine and bone. Biochem. Med. 1952, 3 (1), 23–30.

11. Lindy S., Halme J., Turto H. Collageno­lytic activity in rheumatoid synovial tissue. Clin. Chim. Acta. 1973, 47 (2), 153–157.
https://doi.org/10.1016/0009-8981(73)90310-0

12. Kliatskin S. A., Lifshits R. I. Lab. delo. 1989, N 10, P. 51–53. (In Russian).

13. Kamyshnikov V. S. Handbook on clinical and biochemical studies, and laboratory diagnosis. Мoskva: Medpres-inform. 2004, 911 p.  (In Russian).

14. Osadchuk M. A., Kapustin V. M. Protein-bound hydroxyproline plasma in acute viral hepatitis. Lab. delo. 1987, N 7, P. 8–15. (In Russian).