ISSN 2410-776X (Online),
ISSN 2410-7751 (Print)
"Biotechnologia Acta" v. 6, no. 5, 2013
https://doi.org/10.15407/biotech6.05.079
Р. 79-86, Bibliography 27, Ukrainian
Universal Decimal classification: 543.554.2:577.152.3:547.495.9
1Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, Kyiv, Ukraine
2Educational and Scientific Centre «Institute of Biology» of Kyiv National Shevchenko University, Ukraine
3National University of Food Technologies, Kyiv, Ukraine
Creatinine is one of the most important analytes in up-to-date clinical analysis. Detection of this metabolite in different physiological body fluids is helpful for the estimation of kidney, muscle, and thyreoid disorders. Creatinine is a marker of renal glomerular filtration and is commonly considered as a diagnostic characteristic of the kidney function, the level of which should be controlled to assess the hemodialysis procedure. The experiments were carried out by potentiometric measuring method. A biosensitive element for creatinine detection was created on the basis of highly selective enzyme creatinine deiminase. The enzyme immobilization onto the surface of pH-sensitive field-effect transistor was performed using photopolymer. The creatinine deiminase-based bioselective element was developed. The main analytical characteristics of the developed biosensor were optimized, optimal conditions for the experiments with real samples were found. It was shown that biosensor based on creatinine deiminase is stable. The responses of biosensor were reproducible and liner range was from 0 to 2 mM with detection limit 0,02 mM. Quantitative determination of creatinine concentration in blood serum was elaborated; the data of biosensor measurement were compared with those obtained by the control method, high correlation was shown R = 0,96. A biosensor based on pH-sensitive field-effect transistor and immobilized creatinine deiminase, advantageous for its high sensitivity and selectivity, might be utilized for the quantitative evaluation of creatinine concentration in blood serum of the patients with renal failure as well as for monitoring hemodialysis efficiency.
Key words: creatinine, renal failure, biosensor, creatinine deiminase, pH-sensitive field-effect transistor.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2013
References
1. Sant W., Pourciel-Gouzy M. L., Launay J. Development of a creatinine-sensitive sensor for medical analysis. Sens. Actuators B. 2002, V. 103, P. 260–264.
https://doi.org/10.1016/j.snb.2004.04.104
2. Pandey P. C., Mishra A. P. Novel potentiometric sensing of creatinine. Sens. Actuators B. 2004, V. 99, P. 230–235.
https://doi.org/10.1016/j.snb.2003.11.016
3. Killard A., Smyth M. Creatinine biosensors: principles and designs. Tibtech. 2000, V. 18, P. 433–437.
https://doi.org/10.1016/S0167-7799(00)01491-8
4. Jaffe M. ?ber den Niederschlag, welchen Pikrins?ure in normalem Harn erzeugt. und ?ber eine neue Reaktion des Kreatinins. Z. Physiol. Chem. 1886, V. 10, P. 391–400.
5. Dobberpuhl Mo Y., Dash A. K. A simple HPLC method with pulsed EC detection for the analysis of creatine. J. Pharmaceut. Biomed. Anal. 2003, 32 (1), 125–132.
https://doi.org/10.1016/S0731-7085(03)00028-1
6. Mer?s I. D., Mansilla A. E., G?mes J. R. Determination of methotrexate, several pteridines, and creatinine in human urine, previous oxidation with potassium permanganate, using HPLC with photometric and fluorimetric serial detection. Anal. Biochem. 2005, 346 (2), 201–209.
https://doi.org/10.1016/j.ab.2005.07.038
7. George S. K., Dipu M. T., Mehra U. R. Improved HPLC method for the simultaneous determination of allantoin, uric acid and creatinine in cattle urine. J. Chromat. B. 2006, 832 (1), 134–137.
https://doi.org/10.1016/j.jchromb.2005.10.051
8. Samanidou V. F., Metaxa A. S., Paradoyannis I. N. Direct simultaneous determination of uremic toxins: Creatine, creatinine, uric acid, and xanthine in human biofluids by HPLC. J. Liq. Chromatogr. A. 2002, 25 (1), 43–57.
https://doi.org/10.1081/JLC-100108538
9. Yokoyama Y., Horikoshi S., Takahashi T., Sato H. A Low-capacity cation-exchange chromatography of ultraviolet-absorbing urinary basic metabolites using a reversed-phase column coated with hexadecylsulfonate. J. Chromatogr. A. 2000, 886 (1–2), 297–302.
https://doi.org/10.1016/S0021-9673(00)00520-3
10. Yokoyama Y., Tsuji S., Sato H. Simultaneous determination of creatinine, creatine, and UV-absorbing amino acids using dual-mode gradient low-capacity cation-exchange chromatography. J. Chromatogr. A. 2005, 1085 (1), 110–116.
https://doi.org/10.1016/j.chroma.2005.01.030
11. Burke D. G., MacLean P. G., Walker R. A. Analysis of creatine and creatinine in urine by capillary electrophoresis. J. Chromatogr. B. 1999, 732 (7), 479–485.
https://doi.org/10.1016/S0378-4347(99)00318-7
12. Tran T. C., Huq T. A., Kantes H. L. Determination of creatinine and other uremic toxins in human blood sera with micellar electrokinetic capillary electrophoresis. J. Chromatogr. B. 1997, 690 (8), 35–42.
https://doi.org/10.1016/S0378-4347(96)00413-6
13. Pobo?y E., Radomska A., Koncki R., G??b S. Determination of dialysate creatinine by micellar electrokinetic chromatography. J. Chromatogr. B. 2003, V. 789, P. 417–424.
https://doi.org/10.1016/S1570-0232(03)00075-8
14. Yao T., Kotegawa K. Simultaneous flow-injection assay of creatinine and creatine in serum by the combined use of a 16-way switching valve, some specific enzyme reactors and a highly selective hydrogen peroxide electrode. Anal. Chem. Acta. 2002, 462 (9), 283–291.
http://dx.doi.org/10.1016/S0003-2670(02)00343-4
15. Costa A. C. O., Costa J. L., Tonin F. G. Development of a fast capillary electrophoresis method for determination of creatinine in urine samples. J. Chromatogr. A. 2007, V. 1171, P. 140–143.
https://doi.org/10.1016/j.chroma.2007.09.029
16. Zinellu A., Sotgia S., Zinellu E. Assay for the simultaneous determination of guanidinoacetic acid, creatinine and creatine in plasma and urine by capillary electrophoresis UV-detection. J. Sep. Sci. 2006, 29 (5), 704–708.
https://doi.org/10.1002/jssc.200500428
17. Tuma P., Samcova E., Balinova P. Determination of 3-methylhistidine and 1-methylhistidine in untreated urine samples by capillary electrophoresis. J. Cromatog. B. 2005, 821 (1), 53–59.
https://doi.org/10.1016/j.jchromb.2005.04.006
18. Rodrigues J., Berzas J. J., Casta?eda G. Very fast and direct capillary zone electrophoresis method for the determination of creatinine and creatine in human urine. Anal. Acta. 2004, 521 (1), 53–59.
https://doi.org/10.1016/j.aca.2004.05.058
19. Zinellu A., Caria M. A., Tavera C. Plasma creatinine and creatine quantification by capillary electrophoresis diode array detector. Anal. Biochem. 2005, 342 (2), 186–193.
https://doi.org/10.1016/j.ab.2005.01.045
20. Kvasni?ka F., Vold?ich M. Isotachophoretic determination of creatinine in meat and meat products. Electrophoresis. 2000, V. 21, P. 2848–2850.
https://doi.org/.1002/1522-2683(20000801)21:14<2848::AID-ELPS2848>3.0.CO;2-N
21. Hu?kova R., Chrastina P., Adam T., Schneiderka P. Determination of creatinine in urine by tandem mass spectrometry. Clin. Chim. Acta. 2004, V. 350, P. 99–106.
https://doi.org/10.1016/j.cccn.2004.07.007
22. Satoh W., Hosono H., Yokomaku H. Integrated Electrochemical Analysis System with Microfluidic and Sensing Functions. Sensor. 2008, V. 8, P. 1111–1127.
https://doi.org/10.3390/s8021111
23. Hsiue G., Lu P., Chen J. Multienzyme-immobilized modified polypropylene membrane for an amperometric creatinine biosensor. J. Appl. Polym. Sci. 2004, V. 92, P. 3126–3134.
https://doi.org/10.1002/app.20229
24. Kukla O., Pavluchenko O., Goltvyanskii Yu. Sensor arrays based on differential ISFET elements for monitoring toxic substances of natural and artificial origin. Sensor Electr. Microsyst. Technol. 2008, V. 2, P. 58–68.
25. Pavluchenko A., Kukla A., Goltvyanskii Yu. Study of stability of characteristics of pH-sensitive field-effect transistors. Optoelectr. Semicond. Techn. 2010, V. 45, P. 90–99.
26. Otto M. Modern methods of analytical chemistry. Ed. Garmash A. V. Moskva: Technoshpere. 2003, V. 1, P. 36.
27. Berezov T., Korovkin B. Biological chemistry. M: Medicine. 1998, P. 567–591.