Biotechnologia Acta


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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

"Biotechnologia Acta" V. 10, No 6, 2017
Р.  45-52, Bibliography  6, English
Universal Decimal Classification: 579.61


I. V. Pereima, T. V. Ivanova

National University of Life and Environmental Science of Ukraine, Kiyv

The purpose of this study was to evaluate the optimal nutritional conditions for macromycetes micelles of the Pleurotaceae family. The factors influencing mycelium growth on the example of two species of Pleurotus were investigated.
To determine the effects of nutrient components five variants of agar medium were used, containing:potato, sweet potato, yam, oak bark and malt extractswith the addition of peptone and glucose. The pure culture of Pleurotus species, in the form of agarics blocks, was placed on the nutrient media. Hyphae germination was observed on the eighth day of incubation.

The temperature influence was evaluated in the range from 16 to 36 °C. The maximum growth of mycelium of both species was observed at a temperature of 28 °С.

It was determined that nutrient media containing potato, sweet potato, yam and malt extracts with the addition of glucose and peptone were the most favorable for growth of mycelium of oyster mushroom. They all had mandatory presence of a source of carbohydrates — glucose. At the same time, the media containing extracts of oak, yam, and partially of potato extract, were optimal for the growth of maple oyster. According to the experiment, the mycelial growth efficiency of the maple oyster was much higherin comparison to the oyster mushroom 1.

Key words: Pleurotus, nutrient media, mycelium.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2017

  • References
    • 1. Cheng Z. Effects of carbon sources, nitrogen sources and minerals on mycelial growth of Cryphonectria parasitica. Afr. J. Agric. Res. 2013; V. 8, P.4390–4395.

      2. Ivanova T. V., Otkidach I. S., Kuzіomko N. O., Zarutskaya A. V., Mamontova A. A., Yuronka K. V., Melnychuk M. D. Nutrient media for a pure culture of mushroom of the genus pleurotus obtaining in vitro. Biotechnol. acta. 2016, V. 9, P. 82–86.

      3. Bai Yh. Optimization for betulin production from mycelial culture of Inonotus obliquus by orthogonal design and evaluation of its antioxidant activity. J. Taiwan. Inst. Chem. Eng. 2012, V. 43, P. 663–669.

      4. Ivanova T. New approaches extraction of viral RNA from edible mushrooms. Scientific Journal «ScienceRise». 2015, 1(15), 44–46.

      5. Badu M. Effects of lignocellulosic in wood used as substrate on the quality and yield of mushrooms. Food. Nutr. Sci. 2011, V. 2, P. 780–784.

      6. Choi I. Physiological characteristics of green mold (Trichoderma spp.) isolated from oyster mushroom (Pleurotus spp.). Mycobiology. 2003, V. 31, P. 139–144.


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