Biotechnologia Acta

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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

"Biotechnologia Acta" V. 9, No 3, 2016
https://doi.org/10.15407/biotech9.03.061
Р. 61-68 , Bibliography 28, English
Universal Decimal Classification: 577.21+575.22+633.16

SSR ANALYSIS IN THE STUDY OF GENETIC DIVERSITY AND SIMILARITY OF BARLEY CULTIVARS

O. R. Lakhneko 1, B. V. Morgun 1, R. M. Kalendar 2, A. I. Stepanenko 1, A. V. Troianovska 3, O. I. Rybalka 3

1 Institute of Cell Biology and Genetic Engineering of the National Academy of Sciences of Ukraine, Kyiv
2 Republican State Enterprise ”National Center for Biotechnology”,  Astana, Kazakhstan
3 Plant Breeding and Genetics Institute – National Center of Seed & Cultivar Investigation of the National Academy of Agrarian Sciences of Ukraine, Odesa

The aim of research was to develop an evaluation system of the genetic polymorphism for barley cultivars of Ukrainian and foreign origin based on the analysis of simple sequence repeats and valuable agricultural trait loci as well as to compose the molecular genetic passports for those cultivars. PCRs with the following separation of amplification products by agarose and polyacrylamide electrophoresis were performed to find out genetic polymorphism. Unweighted Pair Group Method with Arithmetic Mean was used for phylogenetic relationship detection. The dandrogram of phylogenetic relationships of 55 barley cultivars was constructed and molecular genetic passports were developed. Molecular genetic passports can be involved in verification for the compliance with standard cultivars, stability and seed purity.

Key words: Hordeum vulgare, SSR markers, PCR analysis, molecular genetic passports.

© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2016

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