Biotechnologia Acta

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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

Biotechnologia Acta
V. 8, No 2, 2015

"Biotechnologia Acta" V. 8, No 2, 2015;  
doi: 10.15407/biotech8.02.052
Р. 52-57, bibliography 15, English
Universal Decimal classification: 577.21+604.6:582.983.2+631.348

COMPARATIVE MOLECULAR GENETIC ANALYSIS BETWEEN UKRAINIAN AND EU REGISTERED GLYPHOSATE-TOLERANT RAPESEED TRANSGENIC PLANTS

A. M. Taranenko, L. O. Sakhno, B. V. Morgun, M. V. Kuchuk

Institute of Cell Biology and Genetic Engineering of the National Academyof Sciences of Ukraine, Kyiv

The purpose of research was to analyze 10 developed at the Institute of Cell Biology and Genetic Engineering lines of rapeseed to confirm the presence and functionality of the transferred transgene CP4 epsps, as well as the differences among those lines from registered transformation events GT73 and GT200 (Monsanto).

During the study extraction of total rapeseed DNA, PCR analysis, electrophoretic separation and visualization of amplicons in agarose gel were conducted, as well as testing of green plants for resistance to glyphosate in greenhouse. The structural difference among 7 transgenic lines from registered transformation events GT73 and GT200 was revealed. Plants showing the presence of synthetic CP4 epsps sequence were resistant to the herbicide in a closed soil. The uniqueness of the obtained transformation events was confirmed, as well as the prospect of using them in breeding.

Key words: rapeseed, glyphosate, genetic transformation, CP4 epsps gene.

© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2008

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