Biotechnologia Acta


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ISSN 2410-776X (Online),
ISSN 2410-7751 (Print)

Biotechnologia Acta
V. 8, No 1, 2015

Biotechnologia Acta, V. 8, No 1, 2015; 
doi: 10.15407/biotech8.01.056
P. 56-62, Bibliography 19, English
Universal Decimal classification: 58.085.2:582.542.11:631.811.98


I. R. Gorbatyuk 1, A. V. Bavol 1, 2, A. V. Holubenko 1, 3, B. V. Morgun 1, 2

1 Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, Kyiv
2 Institute of Plant Physiology and Genetics, National Academy of Science of Ukraine, Kyiv
3 Taras Shevchenko National University of Kyiv, Ukraine

The aim of the study was to determine the dependence of morphogenetic reactions of wheat callus tissues to content of syntetic growth regulators of auxin nature (picloram, dicamba) in the nutrient medium.

Apical meristems of Triticum aestivum wheat were the primary explants for callusogenesis. Basic culture medium MS supplemented by vitamins of Gamborg, dicamba at different concentrations (0.2, 0.4, 0.6 mg/l), and picloram (0.16; 0.25; 0.5 mg/l) was used for regeneration. It was established that dicamba at a concentration of 0.2 mg/l is the most effective for production of regenerants. It was also observed that at the concentration of 0.16 mg/l picloram there are the formation of the greatest number of morphogenic zones (60%) and a significant amount of plant-regenerants. Increased concentrations of picloram to 0.25 mg/l and 0.5 mg/l caused a decrease in the number of morphogenic islands: in the first case, 10%, and the second – 36.4%. Among the described options the MS medium supplemented with 0.5 mg/l 6-benzylaminopurine and 0.16 mg/l picloram was the most effective. Shoots obtained from callus culture were capable to form roots in vitro and adapt to septic conditions. Regenerated plants when cultivated in greenhouse showed high viability (over 75%) and reached the generative phase.

Key words: growth regulators, picloram, dicamba, Triticum aestivum, in vitro culture.

© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2008

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