Biotechnologia Acta

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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

"Biotechnologia Acta" V. 7, No 6, 2014
doi: 10.15407/biotech7.06.075
Р. 75-82, Bibliography 9, Ukrainian
Universal Decimal classification: 577.21+633.16+663.421+544.475

DISTRIBUTION OF ALLELES OF HvITR1 GENE ENCODING
CMe (BTI-CMe) TRYPSIN INHIBITOR WHICH RELATED WITH COLLOIDAL STABILITY OF BEER AMONG BARLEY VARIETIES REGISTERED IN UKRAINE

А. I. Stepanenko1, B. V. Morgun1, 1O. V. Stepanenko 1, S. S. Polishchuk2, O. I. Rybalka1

1Institute of Cell Biology and Genetic Engineering of the  National Academy of Sciences of Ukraine, Kyiv
2The Plant Breeding and Genetics Institute —  National Center of Seed and Cultivar Investigation of the National Academy of Agricultural Sciences, Odesa, Ukraine

The  biotechnological  analysis  of  modern barley  varieties  of  national  and  foreign  origin for the presence of valuable SE-ve genotypes was done.  Identification  of  the  allelic  status  of  the gene  was  performed  among  109  barley  samples using  the  developed  multiplex  PCR  system  for SNAP  molecular  markers.  Among  the  studied varieties SE+ve allele was identified in 75 barley samples  and  the  other  contained  SE-ve allele. 6 samples which revealed both alleles SE+ve and SE-ve were heterogeneous (+/-).

The  obtained  results  were  compared  with the data of the State register of plant varieties suitable for dissemination in Ukraine and showed that  8  samples  referred  to  as  forage,  contained SE+ve allele and none of them were SE-ve allele. Of 38 varieties, which quality was identified as suitable for brewing, 19 contained SE+ve allele, the rest — allele SE-ve. The results of performed analysis  of  the  spring  barley  collection  for allelic composition of HvITR1 gene are of great practical importance as for the correct selection of  mating  pairs  for  malting  breeding,  for  the selection of elite plants in breeding populations, or  evaluation  of  barley  seeds  for  beverage brewing purposes.

Key words: barley  biotechnology,  haze  beer, СМе protein.

© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2008


 

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