ISSN 1995-5537

"Biotechnology" journal V. 4, No. 4, 2011
Р101-103, Bibliography 8, Russian.
Universal Decimal classification: 577.151.042: 577.152.311

THE METHOD FOR IDENTIFICATION
OF HORSE SERUM BUTYRYLCHOLINESTERASE

Yu. G. Zhukovskii, V. A. Zhukovskaya, L. P. Kuznetsova, O. R. Nikitina, O. O. Sochilina

Sechenov Institute of Evolutionary Physiology and Biochemistry
of the Russian Academy of Sciences, St. Petersburg

In the industrial production of cholinesterase and in laboratory practice,  sometimes it needs to identificate these enzymes. It might be due to violations of labeling in enzyme production and control and storage of the readymade products as well.

A method for identification of horse serum butyrylcholinesterase out of cholinesterases from other biological sources such as acetylcholinesterases of the electric eel and numbfish electric tissues, out of the human, horse and camel erythrocytes; butyrylcholinesterases — of the pigeon blood serum and pigeon brain tissue; propionylcholinesterases — out of the serum and the brain tissue was suggested. The method is based on different sensitivity of cholinesterase domen (catalytic centre) of there enzymes to the two reversible phosphonium inhibitors: (С₆Н₅)₃Р⁺– СН₃·I^– (1) and (С₆Н₅)₃Р⁺– СН₂СН₂СН₃·Br^– (2). For this purpose inhibitor concentration (C₁ is for inhibitor 1 and C₂ is for inhibitor 2) decreasing enzymatic cleavage of each cholinesterase twofold was determined and then the value of relation С₁/С₂ was calculated. The identified cholinesterase was horse serum butyrylcholinesterase if the value С₁/С₂ was higher than 4.

.Key words: acetylcholinesterase, butyrylcholin esterase, propionylcholinesterase, phosphonium inhibitors.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008